In Vitro Antioxidant Activities of Six Lactic Acid Bacteria Isolated from Human Intestinal Tract
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Abstract:
The antioxidant activities of 6 lactic acid bacteria isolated from the intestinal tract of the longevity village people of Jiangsu Rugao were studied by hydrogen peroxide tolerance assay, hydroxyl radical and DPPH radical scavenging assays, reducing activity assay, anti-lipid peroxidation assay, T-SOD and GSH-Px activity assays in this study. The results showed that L. fermentum L2, L. fermentum L4 and E. faecium E2 had higher resistant capability against 1.0 mM hydrogen peroxide than the others. Different constituents of 6 lactic acid bacteria had different antioxidant activities, among which the antioxidant activity of fermentation supernatant was superior to bacterial cell and intracellular extract. The fermentation supernatants of L. fermentum L2 showed the highest hydroxyl radical scavenging activity and T-SOD activity, while the fermentation supernatants of E. faecium E2 demonstrated the highest DPPH radical scavenging activity and GSH-Px activity. In addition, the fermentation supernatants of L. fermentum L1 had the highest reducing power; while the fermentation supernatants of L. casei L3 had the highest lipid peroxidation-inhibitory activity. Six lactic acid bacteria had good antioxidant capacity and could be used in future as potential probiotics.
