Collagen Fibers Morphology and Physical and Chemical Properties of Collagen of Sea Cucumber
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Abstract:
Collagen is the main component of sea cucumber body wall, and the study of its physical and chemical properties will provide theoretical basis for processing sea cucumber. The characteristics of collagen fibers of sea cucumber were analyzed by histological method and sea cucumber collagen was extracted by pepsin-solution method for analysis of their physical and chemical properties. Sea cucumber body wall was mainly composed of collagen fibers which exhibited reticular arrangement, and little myofibril was found. Ultraviolet-visible absorption spectra exhibited a maximum absorbance at 235 nm, but little absorbance near to 280 nm. FT-IR demonstrated the existence of triplehelix structure. SDS-PAGE pattern showed that the structure of sea cucumber collagen was (α1)3 which was absence of disulphide bonds, and the molecular weight of α1 chain was about 135 kDa. Amino acid composition analysis indicated that the content of glycine was high (33.3%), and the level of proline and hydroxyproline was 16.4%, in accordance with the aquatic collagen characteristics. The shrinkage temperature (Ts) of sea cucumber collagen was 67.56 ℃, higher than that of pollock skin collagen, and the denaturation temperature (Td) was 22.3 ℃, similar to that of pollock skin collagen.
