Simultaneous Determination of 10 Synthetic Colors in Drink by Solid-Phase Extraction Coupled with HPLC/Photodiode Array Detection
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
A method for simultaneous determination of 10 synthetic colors (new red, tartrazine, amaranth, indigotin, carmine red, sunset yellow, allura red, brilliant blue, acid orangeⅡ and erythrosine) in drink by solid-phase extraction coupled with high performance liquid chromatography-photodiode array detector was developed. The drink samples were cleaned up by Waters Oasis WAX and separated on an Aglient TC-C18 (5 μm, 250 mm×4.60 mm) column with methanol-0.02 mol/L ammonium acetate as mobile phase by gradient elution. The wave lengths for detection were from 210 nm to 650 nm. Qualitative analysis of synthetic colors was based on their retention times and ultraviolet absorption spectra. External standard method was applied for quantitative analysis. The results showed that the linear ranges of 10 synthetic colors were 1~20 μg/mL (r＞0.999). The recoveries ranged from 90.2% to 101.6%with RSDs of 0.44~2.48% (n=6).The detection limits were in the range of 0.01~0.02 mg/kg. The method was simple, accurate and sensitive, which was suitable for the simultaneous determination of synthetic colors in drink.
