Abstract:Cronobacter spp. are emerging opportunistic pathogens. So far, little is known about the species’ immunogens and potential virulence factors. Immunogenic proteins play an important role in the pathogenicity of bacteria. In this study, the immunogenic proteins of C. sakazakii ATCC 29544 was identified using immunoproteomics. The polyclonal antibody was prepared using the formaldehyde-fixed C. sakazakii ATCC 29544 as antigen. Then the proteins of C. sakazakii ATCC 29544 were extracted using sucrose density gradient centrifugation and separated using two-dimensional electrophoresis. Western blot was used to detect the surface-exposed proteins and mass spectroscopy was used to identify them. 7 immunoreactive proteins were identified in this study.
