Extraction of Resveratrol from Polygonum cuspidatum
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Abstract:
Resveratrol is an important ntural compound with bioactivity. In this experiment, Polygonum cuspidatum was used as raw materials for extraction process f resveratrol. Effects of solid-liquid ratio, raw material particle size, ethanol concentration, extraction time, extraction temperature were investigated on the extraction rate. The orthogonal test was used to determine the optimum conditions with solvent and enzymatic extraction of resveratrol in Polygonum cuspidatum. The results showed that the best solvent extraction conditions were pH 5, solid-liquid ratio 1:65 (g:mL), temperature 60 ℃ and extraction time 120 min; The optimum conditions for enzymatic extraction were pH 6.0, solid-liquid ratio 1:40 (g:mL), hydrolysis temperature 60 ℃, and hydrolysis time 120 min. The results showed that, the yields of solvent and enzymatic extractionwere 0.496% and 0.942%, respectively. Compared with solvent method, extraction yield of resveratrol by using enzymatic method was increased by about 2 times. Enzymatic method can be used as the preferred method of extraction of resveratrol.
