A New Method for Enzymatic Synthesis of Xylose Diester in Two-solvent Medium and its Structural Identification
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Abstract:
A new method for synthesis of xylose esters based on solvent strategy was developed in this paper. Binary organic solvent of n-hexane and pyridine (25:75, V/V) was used as reaction system to well dissolve sugar substrate and meanwhile maintain the enzyme activity. Immobilized lipase CAL type B, Novozyme 435, was chosen as a biocatalyst to synthesize xylose ester via acylation of xylose and vinyl methacrylate. HPLC analysis showed that the highest substrate conversion (>90%) was obtained when the ratio of xylose to vinyl methacrylate, shaking speed and reaction temperature were 1:10, 150 r/min and 30 ℃, respectively. The products were structurally identified by GC-MS and 13C-NMR as a diester of xylose, 1′, 5′- dimethacrylic xylose ester. This research provided an efficient and environmentally-friendly method with application potential in production of amphoteric compounds used in food, chemical and papermaking industrils.
