Abstract:The dynamic variation of microbial flora in kefir liquor during its fermentation was studied by PCR-DGGE technique. The analysis result showed that 3 different visible bands in bacteria community and only 1 band in yeast community were obtained in their electrophoresis pattern. Anaylsis of 16SrDNA sequence blast showed that these sequences of all 3 different visible bands had a high homology with the sequence of 16S rDNA of lactic acid bacteria (LAB). They belonged to Leuconostoc mesenteroides, Lactobacillus kefiranofacien and Lactobacillus kefir. The sequence of the band in yeast community had a high homology with the 16S rDNA of yeast Torulaspora delbrueckii. Yeast Torulaspora delbrueckii was, for the first time, found in kefir grains.
