Rapid Determination of Organic Acids and Vitamin in Ju’lu Apricot by a Multi-wavelength Analysis
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Abstract:
High performance liquid chromatography (HPLC) with diode array detector (DAD) was applied to determine water-soluble organic acids (malic acid, citric acid and succinic acid) and vitamins (Vc, VB1 and VB2) in apricots sampled from Julu. The apricots samples were eluted using 0.5 mmol/L H3PO4 -acetonitrile as mobile phase at several wavelengths (210 nm, 254 nm and 270 nm) . The substances can be efficiently separated in 13 min with RSD value ranged in 0.75~2.12%. The precisions were between 0.94% and 1.55% and the result of recoveries was of 96.70~101.9%. The difference in organic acid and vitamin contents between Julu apricot and those sampled from other sources were also compared.
