Extraction and Antioxidant Activity of Polyphenols from Pear
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Abstract:
This study was designed to investigate the extraction of free and bound phenol from the peels and the flesh of pear by solvent extraction method. The antioxidant activity of the peels and the flesh was also studied using the DPPH? assay. The results showed that the total phenol in peels was 201.26±6.90 mg gallic acid equivalents per 100 g fresh pear peels, including 135.36±1.57 mg soluble free phenol and the 42.46±3.05 mg bound phenol. And the total phenol in the flesh was lower with the value of 23.73±2.28 mg gallic acid equivalents per 100 g fresh pear flesh, including 12.54±0.98 mg soluble free phenol and 11.19±1.30mg bound phenol. The content of the flavonoid had a positive correlation with the content of the total phenol. Comparing the antioxidant activities of the peels and fresh, the free phenol in pear flesh had the highest antioxidant activity, with the values of DPPH? scavenging rate EC50 and AE being of 248.59 μg/mL and 0.0040, respectively. The bound phenol in the pear fresh had the lowest antioxidant activity, with the values of DPPH? scavenging rate EC50 and AE being of 1043.24 μg/mL and 0.0010, respectively.