Evaluation of the Preservation Methods for Two Microalgal Concentrates
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Abstract:
Various cryoprotectants and cooling methods were adopted for 3-month preservation of the concentrates of Nannochloropsis oculata (2×109 cells/mL,at 4 ℃ and -18 ℃) and Spirulina platensis (7% dry weight at -18 ℃). The results showed that, the highest relative cell activity of N. oculata concentrates preserved for 3 months reached 48.17% of that control (fresh cultured cells) by fast freezing at -18 ℃ without cryoprotectant, which higher than other treatments significantly (P<0.05). Under the same conditions, the loss of protein and EPA in the concentrates of N. oculata had were 3.42% and 2.73%, respectively,The lose of the protein and total fat acids in the concentrates of S. platensis were 4.17% and 19.33%, respectively. Based on the systematic comparison, the method of fast freezing at -18 ℃ without cryoprotectant was determined as the favorable for preservation of the concentrates of N. oculata and S. platensis at low temperature.
