Simultaneous Determination of Residual Carbendazimin and Thiophanate-methyl in Ginseng by High Performance Liquid Chromatography
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Abstract:
A High performance liquid chromatography (HPLC) method was developed in this paper for simultaneous determination of residual carbendazimin and Thiophanate-methyl in Ginseng, a Chinese traditional medicine.The HPLC analysis of the samples were carried out using methanol-water as mobile phase on a Diamonsi C18 column at 285 nm. The average recovery and RSD for Carbendazimin were 85.73% and 2.07% (n=6), respectively. The average recovery and RSD for Thiophanate-methyl were 85.83% and 2.39% (n=6), respectively. The standard curve of carbendazim was linear in the range of 0.2~1.0 μg (R=0.9990)and the standard curve of Thiophanate-methyl was linear in the range of 0.2~1.0 μg (R=0.9993). This method was simple, rapid and reliable1, which is suitable for the determination and safe control of residual carbendazimin and Thiophanate-methyl in Chinese traditional medicines.
