Determination of Polyphenols and Anthocyanins Contents in Mulberry Tablet
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Abstract:
The Folin-Ciocaiteu method was used to determine the total polyphenol content of mulberry tablet. The main monomer and contents of anthocyanins were determined by HPLC. The pH differential method was applied to determine the total anthocyanins content. The total polyphenol content of Lipid-lowering tablets was 63.76 mg GAE/g and the total anthocyanin content was 6.63 mg/g. The monomer of cornflower-3-glucoside and cornflower-3-rutin were the main anthocyanin compounds with thire contents being of 2.91 mg/g and 2.55 mg/g, respectively. And the ratio of the two compounds was 1.14:1. A better linear relation was found in the range of 2~500 μg/mL (R2=1.0000~0.9999) and the average recovery rate was in the range of 102.5~97.8% with the RSD of 0.93~1.01%. The method applied in this research was sensitive, accurate and reproducible, which can be effectively used in the quality control of mulberry tablet.
