Identification of Adulteration in Walnut Milk Containing Soybean Milk
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Abstract:
A method for detection of adulteration in walnut milk containing soybean milk by high performance liquid chromatography was established. The extraction conditions were sample-methanol ratio 1:9 (V/V), ultrasonic time 20 min,and centrifugal time 20 min at 4000 r/min. The analysis was carried out with a column C18 (150 mm×4.6 mm, 5 μm) using methanol-water as gradient mobile phase at 258 nm. The results showed that walnut milk containing soybean milk was identified effectively through determination of soybean isoflavones. The linear ranges were 0.3~200 μg/mL for daidzin and genistin and 0.1~150 μg/mL for daidzein and genistein, respectively. The linear correlation coefficients were between 0.9968 and 1.0000. And the averrage recoveries were between 80.73% and 116.44%. The relative standard deviations (RSDs) were less than 4.62% (n=5) and the detection limits were 0.03~0.1 μg/mL. This method was fast, sensitive and simple.
