Identification of 12 Soybean Isoflavones in Soybean Meal by High Performance Liquid Chromatography-Electrospray Ionization-Multi-stage Tandem Mass Spectrometry
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Abstract:
To determine the construction of soybean isoflavone, high performance liquid chromatography-electropray ionization multi-stage tandem mass spectrometry（HPLC-ESI-MSn） was successfully applied to analysis and determine ethanol extracts of soybean meal. Thouth this method, the accurate molecular ion peak and multistage fragment ion information were provided. Relative molecular mass, retention time and constructions of flavoneid aglycones of 12 kinds of soybean isoflavones were analyzed. The types of glycogen in isoflavone were also discussed. The 12 kinds of soybean isoflavone glycosides were identified as daidzein, glycitein, genistein, daidzin, glycitin, genistin, 6"-o-acetyldaidzin, 6"-o-acetylglycitin, 6"-o-acetylgenistin, 6"-o-malonyldaidzin, 6"-o-malonylglycitin and 6"-o-malonylgenistin.
