Two-step Enzymatic Hydrolysis for Preparation of Peptides from Sunflower (Helianthus annuus L.) Protein Isolates
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Abstract:
Two-step enzymatic hydrolysis reactions were investigated to prepare peptides from sunflower protein isolates. In order to maximize content of peptides and optimal degree of hydrolysis (DH), crucial hydrolysis parameters were optimized. Results showed that the maximum content of peptides of 4.249 mg/mL was obtained under the following conditions in the first enzymatic step: Alcalase 2.4 Ldosage 1.0 ?10-2g/g , hydrolysis time 104 min, temperaure 55 ℃, pH 8.5, and substrate concentration 5?10-2 g/mL. In the second enzymatic step, the optimum conditions were,: Flavourzyme dosage 0.44?10-2 g/g, reaction time 120 min and pH 7.5. Unde the optimum condition, the DH was 30.97%. In addition, much higher peptide contents than using single enzyme method was found when using this two-step enzymatic method.
