In this paper, the activity of Candida Rugosa lipase (CRL) in catalyzing hydrolysis reaction was investigated through single factor experiment. And the optimal reaction conditions for CRL catalyzed hydrolysis were obtained as follows: temperature 55 ℃, buffer pH 6.0, and enzyme concentration 100 mg/mL. Under these conditions, the activity of 100 mg/mL CRL was 39.78 mU. CRL was suitable for catalysis at temperatures below 60 ℃and was stable when stored in buffer solution at pH values of 5.0~7.0.
