Optimization of Fermentation Conditions for the Produciton of Amylase and Protease by a Strain of Aspergillus niger
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Abstract:
A strain A020 of Aspergillus niger with high productivity of amylase and protease was isolated. The optimum compositions of fermentation medium were as followed: 1% peptone, 1% maltose and 3×10-3 mol/L NaCl in banana peel basic medium. And the optimum fermentation conditions were achieved as follows: temperature 30 ℃, initial pH 6.5 and medium volume 150 mL in 500 mL flask. Under these conditions, the activities of amylase and protease and cultured for 4 d at 150 r/min were 1278 U/mL and 918 U/mL, respectively. The amylase and protease activities increased by 68.2% and 41.2%, respectively.
