Two-step Enzymatic Extraction of Banana Resistant Starch
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Abstract:
In this paper, a two-step enzymatic method was used for the extraction of banana resistant starch (RS) by using pectase, cellulose and a-amylase. The results showed that, in the first enzymolysis step, the optimum conditions were mass ratio of pectinase to cellulase of 1:2, enzyme dosage of 0.22%, reaction temperature of 45 ℃, pH value of 5.0 and reaction time of 35min. In the second enzymolysis step, the best extraction conditions were a-amylase dosage of 0.35%, reaction temperature of 52 ℃, pH value of 6.3 and reaction time of 3.5 h. With this method, the extract rates of RS were 81.24%.
