Purification of Peanut Allergen Ara h1 with Anion-exchange Chromatography
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Abstract:
Protien Ara h1 is a major allergen in peanut. In order to obtain the natural peanut allergen Ara h1, ammonium sulfate precipitation and anion-exchange chromatography were used for purification. The analysis and identification of peanut allergens was carried out using SDS-PAGE and Western blot. The results showed that the purity of Ara h1 was more than 90 percent and the recovery accounted 23.1% of total Ara h1 with the anion-exchange chromatography. The Ara h1 purfied by anion-exchange chromatography had immunocompetence. It can combine to peanut allergy patients serum with high specificity.
