The fingerprint of Radix Fici in medicated diet by HPLC was established and the medicinal materials that can be used as evaluation index of medicated diet were explored. Chromatographic conditions of HPLC were as follows：Waters Shield RP C18 column (250 mm×4.6 mm, 5 μm), methanol (A) and 0.1% phosphoric acid (B) as gradient mobile phases, flow rate 1.0 mL/min and detecting wavelength at246 nm, and column temperature 30 ℃. Fingerprint was established with 14 common peaks and determination of Psoralen was the highest. Similarity was in the range of 0.90-0.99 and methodology reached the required standard. Characteristic constituents of Radix Fici could be detected in the medicated diet, which was appropriate for the evaluation index of medicated diet. The impact of fatty acid on detection of characteristic constituents of Radix Fici was small.
