The preparation and screening of antibody of Furazolidone Metabolite
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Abstract:
Using of 4 Furazolidone Metabolite Residues, immunogen and coating antigen were synthesized. Then, 4 immunogen were used to immunize 2 New Zealand rabbits. 8 antiserums were obtained and purified. Using indirect ELISA method, the OD value which was higher than that of negative samples of serum were filtered out and used in subsequent experiments, and then antibody titer were determined. The results implied when coating antigen A and B coated microtiter plates. No.7 and No.8 antiserums had a good affinity on the coating antigen. The derivatives of Furazolidone Metabolite had a good effect on antibody inhibition. Through the determination of antibody titer, No.7 and No.8 antiserums both had high titer and high specificity features, of which NO.8 antiserum provided higher antibody titer and higher affinity. Through further screening of 2 coating antigen and 2 antiserums, coating antigen B and No.8 antiserum were chosed for ELISE method.
