The Medium Optimization of Rhizopus sp. for Bioconversion of Canrenone
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Abstract:
With the conversion of canrenone for index, the nutritional requirements of the Rhizopus sp. UJS-0602 were evaluated to optimize the biotransformation of canrenone. Different carbon source, nitrogen source, inorganic salt and some important factors of concentration on the influence of the conversion were evaluated through the shake flask culture and High Performance Liquid Chromatography. The L9 (34) orthogonal experimental design was adopted to confirm the optimum cultivating conditions: glucose concentration 30 g/L, corn steep liquor 25 g/L, yeast extract paste 12 g/L and KH2PO4 concentration1.5 g/L. Under the optimized conditions, the conversion of canrenone was 87.68%, being 9.06% higher than that before optimization.
