Extraction of Effective Components from Schisandra sphenanthera Rehd.Et Wils and their Distribution State in Different Phase System
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Abstract:
Using total Lignans and Polysaccharide of Schisandra sphenanthera Rehd. Et Wils as the indicator, study on the optimal extraction technology conditions of its effective components and distribution in ethanol and water phase. Using UV spectrophotometry and Phenol-Sulfuric acid method for testing, the results showed that the optimal extraction conditions of total lignans were alcohol concentration 70%, liquid ratio 1:9, extraction times 3, and extraction time 2 h, under which the extraction rate reached 9.76 mg/g. The optimal extraction conditions of polysaccharide were alcohol concentration 70%, liquid ratio 1:7, extractio times 3 and extraction time 1.5 h, under which the extraction rate reached 11.32 mg/g. The results also show that, after separation, the extract in ethanol phase was significantly greater than the total Lignans and polysaccharide content in aqueous media content. Total Lignans and polysaccharide content was similar in the ethanol phase. Polysaccharide content was higher than the total lignans content in the water phase.