Effect of UV-B radiation on Reactive Oxygen Species and Antioxidant Enzymes of Nostoc flagelliforme Cells
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Abstract:
In order to explore the impact of ultraviolet-B (UV-B) radiation on reactive oxygen species (ROS) metabolism, the changes of reactive oxygen species(ROS) content and antioxidant enzyme activities were determined in liquid suspension cultivation of Nostoc flagelliforme cells. The results indicated that the superoxide anion (O2-) production and hydrogen peroxide (H2O2) content of Nostoc flagelliforme cells were increased under 1 and 5 W/m2 UV-B treatment，and the maximum of O2- production reached under UV-B treatment for 6 h，that were 480.62 and 510.34 nmol/g DW, respectively. The H2O2 content was increased at all times and reached 210.24 and 229.89 nmol/g DW under UV-B treatment for 48 h .The malondialdehyde (MDA) content attained 0.085 and 0.147 μmol/g DW ,which were 251.70% and 413.72% of the control exposure to UV-B for 48 h . The activities of superoxide dismutase (SOD) and catalase (CAT) reached the maximum at 12 h at both intensities. The ascorbate peroxidase (APX) activity was increased in the early radiation（0~6 h），then APX activity was unstable under 1 W/m2 UV-B treatment and declined rapidly under 5 W/m2 UV-B treatment.
