Comparison of Two Kinds of Vibrio Chromogenic Medium for Identification of Presumptive Vibrio parahaemolyticus
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Abstract:
The presumptive Vibrio Parahaemolyticus strains isolated by thiosulfate - citrate - bile salts - sucrose agar (TCBS) from food samples were preliminary identified by HKM and CHROMagar vibrio chromogenic media according to the National Standards ( GB /T4789.7- 2008) and finally were confirmed by the API and MID identification. In qualitative and quantitative detection of 97 food samples, suspect strains appeared in 109/970 TCBS plates, corresponding to 31 samples. 109 suspect strains from different TCBS plates were inoculated with two kinds of chromogenic media. The results showed that there were 64 purple strains, corresponding to 22 samples. 60 strains were identified as V. parahaemolyticus by API20E among 64 purple strains, corresponding to 22 samples, the positive rate was 22.68%. The sensitivity, specificity, and accordance of two media were 100.00%, 91.84% and 96.33% respectively. HKM and CHROMagar vibrio chromogenic media can be recommended as good choice for preliminary identification of TCBS suspicious strains from foods.
