Abstract:Phyllostachys tianmuensis shoot shell was used as experimental materials to extraction total polyphenols under ultrasound assistance. The effects of four independents variables in terms of ultrasonic power, extract time, ethanol concentration and solid-liquid ration of total polyphenols were studied by response surface methodology. The optimum extraction conditions were as follow: the ultrasonic power 200W, extract time 40.2 min, ethanol concentration 42% and solid-liquid ration 1:50.5. Under these conditions, the predicted maximum yield and average experimental yield were 5.50% and 5.57%, respectively. The relative error between then was 1.27%.