Study on Ultrafiltration Separation and Immunocompetence of Polysaccharides from Antrodia
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Abstract:
Antrodia polysaccharide was separated by ethanol precipitation and ultrofiltrationr (UF) and the effects of Antrodia polysaccharide were investigated on immune function of mice. The result showed that the rate of Antrodia polysaccharide was 18.18% , increased by 56.99% compared to that by ethanol extracting. The yields of Antrodia polysaccharides with molecular weights of >1000 kD (GFP100), 100~1000 kD (GFP10) and 10~100 kD (GFP1) were 3.85%, 1.81% and 4.69%, respectively. All the polysaccharide components separated by UF can significantly increase the proliferation rate of mouse spleen lymphocytes. Conclusion: The method of ultrafiltration separation of Antrodia polysaccharide was simple and feasible.
