Influence and Mechanism of Polyamines Addition on S-adenosyl-L-methionine Synthesis by Pichia pastoris
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Abstract:
S-adenosyl-L-methionine (SAM) was a type of active amino acid required largely. SAM was mainly produced through the biosynthesis reaction within Pichia pastoris cell that overexpressed methionine adenosyltransferase (MAT), while the inducer methanol led to cell death at some extent during cultivation process with high cell density. Thus this study firstly revealed that methanol exerted oxidative stress on P. pastoris cell, and then investigated the influences of adding polyamines (putrescine and spermidine) on SAM biosynthesis and reaction mechanism. The results revealed that polyamines can lessen oxidative damage through elevating catalase activity, and additionally, improving MAT activity and cell growth. As a result, 19.2% higher level of SAM deposit was achieved.
