Rapid Detection of Tetracyclines Residue in Fresh Milk using Quantitative Fluorescent Assay
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
A immunochromatographic method was developed by antibody labeled by fluorescent nanoparticles and using fluorescent immunoassay(FIA) meter of Guangzhou Wondfo Biotech Co., Ltd. With this method, the concentration of four kinds of tetracyclines residue could be detected highly-sensitively with the purpose of determination of total amount of tetracyclines in fresh milk. The results showed that the limit of detection for tetracycline was 10 μg/L and the recovery rates for chlortetracycline, oxytetracycline and doxycycline were more than 70%. The kit had no cross-reactivity with other drugs besides that the cross-reactivity towards streptomycin was 0.32%. The whole detection process could be performed in about 15 minutes. The rapid quantitative determination of total amount of tetracyclines was realized by means of fluorescent quantitative immunochromatographic method developed.
