Analysis on the Content of Chlorogenic Acid from Early-flowering and Later-flowering Lonicera Macranthoides
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Abstract:
To explore suitable planting fine Lonicera macranthoides varieties in north Guizhou, the content of chlorogenic acid in flower buds and leaves of early-flowering and later-flowering varieties were researched. Chlorogenic acid were obtained with ultrasonnic wave-assisted extraction technique, and analyzed using HPLC methods with operating condition as follows: Diamonsil (5 μm, C18(2), 250 mm×4.6 mm),the volume flow 1.0mL/min，the column temperature 40℃ and the detection wavelength 325nm. The mobile phase methanol/acetonitrile (5:11)-0.1% phosphoric acid=20:80. The result showed that the content of chlorogenic acid ranged from 2.36% to 3.44% in flower buds and from 0.49% to 1.11% in leaves of early-flowering Lonicera macranthoides. In flower buds and leaves of later-flowering one, it as in the range of 2.60%~4.07% and 1.92%~2.83%, respectively. It was concluded that the later-flowering Lonicera macranthoide was a rich-chlorogenic acid breeding strain.
