Abstract:In order to improve the yield of Bacterial Cellulose fermented by Acetobacter xylinum CGMCC5173, the optimum conditions for fermentation were investigated. The result showed that, in CJMF medium, BC production reached the highest as 43.91 g/L when A. xylinum 5173 was cultivated to the second generation. In HMF medium, when the quantity of inoculums content, sucrose, sodium acetate, ethanol and L-lactic acid are 10%, 10%, 1.0%, 1.5% and 0.25% respectively, BC production were enhanced up to their peak, as 14.79 g/L, 7.47 g/L, 4.24g/L, 40.07 g/L and 21.92 g/L. However,the higher the D-lactic content was, the less the BC content was found. When the medium was mixed 20% CJMF with 80 % HMF, the BC yield was highest as 29.30 g/L. The results indicated that controlling cultivation generation and using mixed medium of HMF and CJMF can improve the BC yield steadily and effectively.
