Study on Protein Removal Method from Camellia Polysaccharides
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Abstract:
This paper studied a new type of proteins removal method from camellia polysaccharides, hydrochloric acid-ethanol isoelectric point method.On the basis of hydrochloric acid isoelectric point method, the new method adding a certain amount of ethyl alcohol into suspension is more effective than the traditional methods to precipitate proteins. Through single factor experiments and orthogonal test, the optimum condition was found as follows: pH 3.4, ethyl alcohol adding 13% and standing time 3 days, under which protein removal rate and polysaccharides yield reached 92.68% and 61.78, respectively. Compared with the traditional method of trichloroacetic acid (TDA) and Sevage, it was more effective and more safe.
