Generation of a Cronobacter (Enterobacter sakazakii) Mutant Library
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Abstract:
Cronobacter (Enterobacter sakazakii), a pathogen commonly found in powdered infant formula (PIF), is an important food-borne pathogen. At present, very limited information is available regarding the function of genes, virulence factors and the pathogenesis of Cronobacter. In this study, plasmid pTnMod-okm and pBSL180 were used to build Cronobacter random mutant library. Finally, the mutant library of Cronobacter was built based on transposon that contained in pBSL180 plasmid of Escherichia coli WM3064 through Chromogenic technology and antibiotic resistance labeling (kanamycin 50 μg/mL). In subsequence research, gene assignment and finding the similar function gene can be achieve, which layed the foundation for further studying the resistance mechanism and the pathogenesis of Cronobacter.
