Quantification of Sibutramine and Fenfluramine in Slimming Foods by Gas Chromatography-Mass Spectrometry
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Abstract:
A new method for the determinination of sibutramine and fenfluramine in slimming foods by gas chromatography-mass spectrometry with selected ion monitor (GC-MS/SIM) was established in this paper. The sample was extracted by ethanol, then the chromatographic separation was performed on a HP-5 MS capillary column. The conditions of GC-MS were optimized, and the selected ions were m/z 72, 114 and 159. Qualitative assessment was conducted using both retention time and mass spectrum which the complex matrix disturbance in slimming foods sample could avoided.Quantitative assertion was achieved using base peak m/z 114 and 72, respectively. Good linear was acquired within the range of 0.1 mg/L~50 mg/L, while and recovery rate at three spike levers (low, medium and high) were between 93.8% and 97.6%. The limit of detection was 1.0 mg/kg.The method was proved to be simple and accurate, and could be applied to the determination of sibutramine and fenfluramine in slimming foods flavor with satisfactory results.
