Abstract:Using high performance liquid chromatography, transferring component from solid to liquid is a prerequisite for the protection of quantitative results of real value, water-soluble solution to determine the basic method for pre-treatment. Results showed that the chafing dish morphine content were 5.31%, 7.60%, 10.20% detected by HPLC on a C18 column. the use of domestic solid phase extraction column can give recovery rate of 98.7%. The HPLC detection conditions were mobile phase 0.05 mol/L potassium dihydrogen phosphate, 0.0025 mol/L heptane sulfonate aqueous solution - acetonitrile, column temperature 35 ℃, flow rate 1 mL/min, and detection wavelength.
