Analysis of Fatty Acids in Euphausia Pacifica by Gas Chromatograph-Mass Spectrometry
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Abstract:
The total lipids of Euphausia Pacifica were extracted by Folch method, Silica column chromatography method was used to separate total lipids into neutral lipid, glycolipid and phospholipid. The fatty acids were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) after derivatized. The results showed that the content of total lipid was 15.16%, 70.19% was neutral lipid which was the main lipid, phospholipid was 25.15% while glycolipid was 4.66%. 20 fatty acids were identified in the total lipid extracted from Euphausia Pacifica, including 11 unsaturated fatty acids, the total content of EPA and DHA was 23.36%. According to the comparison and analysis of fatty acids in neutral lipid, glycolipid and phospholipid, the compositions and contents of fatty acids had definite difference. This thesis is intended to provide theory basis for nutritional evaluation and optimum exploitation of Euphausia Pacifica.
