Optimization of the Fermentation Conditions for Fibrinolytic Enzyme Produced by Bacillus Amyloliquefaciens by Response Surface
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Abstract:
The fermentation conditions for fibrinolytic enzyme produced by Bacillus amyloliquefaciens was optimized by response surface methodology. Firstly, the impact of single factor on fibrinolytic enzyme production was studied and three factors with the most significant effect on fibrinolytic enzyme production were obtained by Plackett-Burman design. The way to reach the highest fibrinolytic activity area was investigated through the steepest climbing experiments. Different levels of the most three significant factors were optimized using central composite designs and response surface analysis. As a result, the optimal fermentation conditions for fibrinolytic enzyme were the concentration of bacteria peptone 2.25%, concentration dextrin 2.65% and fermentation time 45 h. Under these conditions, the enzyme activity was 96.340 IU/mL. Validation experiment showed the activity of fibrinolytic enzyme was 98.947 IU/mL.
