Determination of Azodicarbonamide in Flour by UPLC-MS/MS
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Abstract:
A quantitative assay for the determination of azodicarbonamide(ADA) using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed. ADA in flour was hydrolyzed with wet heat processing and formed semicarbazide (SEM) finally. SEM was derivatized overnight with 2-nitrobenzaldenhyde at 37 ℃. The derivative was extracted with ethyl acetate, and MS detection was performed with the positive ion mode in multi-reaction monitoring (MRM). Determination of ADA was according to the amount of SEM which was determined as external method. Compared with the method of HPLC, the limit of detection was 0.21 mg/kg for ADA, which was lower than 1mg/kg for HPLC. Within the linear range of 1~100 mg/kg, correlation coefficient was 0.9997. The recovery rate were 86.8~117.2% (RSD <8%). The method developed was convenient, accuracy, and sensitivity to be used effectively for determining ADA in flour.
