Preparation of Chito-oligosaccharides by Enzymatic Hydrolysis of Chitosan Powder
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Abstract:
In order to save the process of colloidal chitosan preparation in hydrolysis of chitosan powder to chito-oligosaccharide techniques. Chitosanse producing strains were isolated and identified as Bacillus cereus D-11. Twenty, forty, sixty and eighty meshes of powder chitosan (3±0.5 cm;D.D. 98.1%) were used as carbon sources to investigate the optimal culture conditions of this strain. The chitooligosaccharides products were analyzed by Thin Layer Chromatography. Compared to control, the enzymatic activity of Bacillus cereus D-11 was increased by 12% and 62.5% in a medium containing 1% Tween 60 or 60 mesh powder chitosan, respectively. The yield of chito-oligosaccharides positively correlated with the enzyme amount. In conclusion, the product of chitosan which was hydrolyzed by chitosanse was determinded as chitooligosaccharides by TLC analysis. The hydrolysis rate of chitoasan by chitosanse was determinded by size of chitosan.