Isolation,Identification and Antimicrobial Activity of Endophyte Fungi from Plant Xanthium sibiricum Patr
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Abstract:
8 strains of endophytic fungi isolated from Plant Xanthium sibiricum Patr were tested with antimicrobial activity assay.6 strains of endophytic fungi showed high inhibitory effects on Alternaria solani,and the inhibition rates were all above 50%. Strain SC008 showed high inhibitory effects on plant pathogenic bacteria and fungi, the inhibition rates of 5 plant pathogenic bacteria and fungi were all above 50%. Strain SC008 was identified by morphological observation and ITS sequence analysis. The result indicated that strain SC008 was Nigrospora Zim. The carbon and nitrogen source assay showed that SC008 could grow fast on the culture medium which carbon source comes from lactose and sucrose, and nitrogen source come from yeast extract and beef extract.