Determination of Chromium VI in the Gelatin by High Performance Liquid Chromatography
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Abstract:
A method for determination of chromium VI in the gelatin by High Performance Liquid Chromatography was developed. Chromium VI was extracted by dipotassium hydrogen phosphate buffer. After filtered, Chromium VI reacted with Diphenylcarbazide to form purple complex. HPLC was applied to the separation and purification of target compound for effectively reducing the disturbance, and the chromiumVI content can be detected selectively by the UV detector. The quantification limit of this method was 0.8 mg/kg. The recovery of chromium VI in gelatin was above 75% in the range of 0.8-50.0 mg/kg. Results indicated that the accuracy and precision met the requirements of the determination of chromium VI content in gelatin.
