Optimization of the Fermentation Medium of a L-lysine-producing Strain by Response Surface Methodology
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Abstract:
The fermentation medium of a L-lysine producing strain Brevibacterium flavum HXL109 to improve the L-lysine production was optimizd. With the software Design Expert, a response surface model was established using the Box-Behnken design(BBD). The effects of glucose, (NH4)2SO4, soybean cake hydrolysate , KH2PO4?3H2O , MgSO4?7H2O, FeSO4?7H2O and MnSO4?H2O on L-lysine yield were analyzed. The results showed that the optimum conditions for HXL109 to produce L-lysine were as follows: glucose 89.48g/L, soybean cake hydrolysate 30.77g/L, (NH4)2SO4 20.89g/L and KH2PO4?3H2O 4.5g/L. Under these conditions, the result of confirmatory experiment (142.65g/L) was in good agreement with the predicted value (143.67g/L). A comparison study showed that the established regression model had good effectiveness in optimizing the fermentation medium of a L-lysine producing strain.
