Oat bran peptiedes were using enzyme technology with the method of single factor experiments and orthogonal experiments. Alkaline protease was found as the best enzyme and the optimum extration conditions were determined as: the ratio of water to materials 18:1,temperature 50 0C, and ,the enzyme dosage 1.0%. After verification, oat peptide extraction rate of this process reached 18.9%. The purity of polypeptide after membrane filtration purification up to 87.4%.