Abstract:In order to further reduce the cost of medium and promote industrialized production,industrialized culture medium of Phaffia rhodozyma for astaxanthin synthesis was studied on the base of pre-optimized culture medium，using cheaper corn flour，corn starch and tomato starch hydrolyzate instead of glucose as carbon source. Shake flask culture and 10 liter fermentor culture were used with the mutant 269 of Phaffia rhodozyma. Results showed that corn starch hydrolyzate could instead glucose as carbon source in the industrialized production of astaxanthin. The optimal hydrolyzed conditions of corn starch were as follows: the concentration of corn starch paste 30% , the concentration of α-amylase 25 U/g starch, the concentration of saccharifying enzyme 200 U/g starch and the saccharifying time 4 hours , under which the cost of culture media could be decrease significantly.
