Extraction and Anti-oxidant Activity Evaluation of Polyphenol from Hempseed Hull
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Abstract:
Hempseed hull, a by-product in hempseed oil processing, is rich in antioxidants. In this study, the conditions of polyphenol extraction from hempseed hull by ultrasonic were optimized on base of yield, and in vitro antioxidant properties of the extract were evaluated. Solvent extraction and macroporous resin absorption were employed in polyphenol separation. The results showed that the optimal extraction conditions were as follows: solid-liquid ratio 1:10, extraction time 30 min, extraction times 3 times and ethonal concentration 60%. HPD-600 was determined as the optimal macroporous resins for hempseed hull antioxidant polyphenol extraction and elunet by 40% ethanol was the target extract with the highest antioxidant activity and yield. The extract presented significant protective capacities of human low density lipoprotein (LDL), protein, and DNA against oxidation evaluated by electrophoresis. The hempseed hull extract was valuable in antioxidant properties and can be utilized as a natural antioxidant dietary supplement.
