With Durian shell as material, dietary fiber was produced via fermentation with Lactobacillus bulgaricus and Streptococcus thermophilus. With single factor experiments and central design experiments, the optimal process conditions were determined as follows: inoculation amount 10.5%, fermentation time 22.0 h and fermentation temperature 38.3 ℃. Under these conditions, the highest productivity (29.50%) could be achieved.