Synthesis and Identification of Hapten and Artificial Antigens of Fenthion
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In order to develop immunoassays to fenthion, an organophosphorus pesticide, one fenthion hapten and two artificial antigens were synthesized before antibody production. The fenthion hapten was produced by combining thiophosphoryl chloride with 3-methyl-4- methylthiophenol and β-alanine. The production was identified through TLC, 1H-NMR and MS. The hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) by the active ester method and the mixed anhydride method, respectively. The products, named as BZB and BGO, were identified through ultraviolet spectrum (UV). A spectrophotometric method using 2,4,6-trinitrobenzene sulfonic acid (TNBS) was performed to estimate hapten to carrier protein ratio. The results showed that the binding rates of BZB and BGO came to 29:1 and 12:1, respectively. Balb/C mice were immunized with BZB and competitive indirect enzyme-linked immunosorbent assay (ELISA) indicated the existence of specific antibodies in the antisera which further verified the success of synthesis.
