Purification of Lipases from Aspergillus oryzae 3.5232 Induced by Tween 80
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Abstract:
The expression of the lipase with 27 kDa molecular weight on cell wall, cell membrane and cytoplasm of Aspergillus oryzae 3.5232 was inducted by Tween 80. Another lipase with 36 kDa molecular weight in cytoplasm was also detected under same conditions. The cytoplasmic lipase was purified to homogeneity by ammonium sulfate precipitaiton and DEAE-Sepharose FF and Sepahdex G-75 column chromatography. Purification fold was 42.5 and the recovery rate was 0.12%. Molecular weight of purified lipase was 36 kDa.
