Determination of Water-soluble Vitamins in Health Foods by UPLC
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Abstract:
Water-soluble vitamins were determiend by Ultra-performance liquid chromatography (UPLC) equipping ultraviolet detector and fluorescence detector in series. Samples were separated by a Acquity UPLC HSS T3 (2.1×100 mm×1.8 μm) column using 0.1% trifluoroacetic acid-acetonitrile as mobile phase in gradient elution mode. Six water-soluble vitamin components in health foods were simultaneously determined, including ascorbic acid (Vc), nicotinamide (B3), thiamine (B1), pyridoxine (B6), folic acid (B11), riboflavin (B2), etc. This method was enabled to achieve wide linear range, well correlation, low detection limit and fast analysis. The results were in accordance with those wtih GB methods.
