Abstract:The purpose of this study was to establish an ultra-sensitive and handy headspace gas chromatographic for the determination of alcohol in rat blood by gas chromatograph. The blood samples containing alcohol and tert-butanol (internal standard, IS) were heated in a water bath. The headspace vapor was drawn into a syringe and injected into a GC port and the experimental conditions of headspace and GC were optimized by taking the peak areas as index. The results showed that the samples heated at 70 ℃for 15 min had a steady peak area and good vapor-liquid equilibrium. Under the optimized conditions, the target peak and the internal standard peak were clearly separated. In the range of 0.05~12 mg/mL, the linear relation between peak area and concentration of the alcohol was found. RSD was 1.63% by testing the standard solution for seven times. The detection limit was 0.005 mg/mL and the reclaim ratio was 93.4%~103.2% (RSD≤3.41%). In conclusion, this method was simple, flexible and effective to detect the alcohol concentration in rats’ blood and can be used widely.
